Enzymatic saccharification of solid residue of olive mill in a batch reactor

N Abdi; F Hamdache; D Belhocine; H Grib; H Lounici; DL Piron; N Mameri

doi:10.1016/s1369-703x(00)00085-1

Enzymatic saccharification of solid residue of olive mill in a batch reactor

Biochem Eng J. 2000 Dec 1;6(3):177-183. doi: 10.1016/s1369-703x(00)00085-1.

Authors

N Abdi¹, F Hamdache, D Belhocine, H Grib, H Lounici, DL Piron, N Mameri

Affiliation

¹ Ecole Nationale Polytechnique d'Alger, 10 avenue Pasteur, B.P. 182, El Harrach, Alger, Algeria

PMID: 11080647
DOI: 10.1016/s1369-703x(00)00085-1

Abstract

This paper describes the enzymatic hydrolysis of solid residue of olive mill (OMRS) in a batch reactor with the Trichoderma reesei enzyme. Before enzymatic saccharification, crude lignocellulosic material is submitted to alkaline pre-treatment with NaOH. Optimum conditions of the pre-treatment (temperature of T=100 degrees C and OMRS-NaOH concentration ratio of about R=20) were determined. The optimum enzymatic conditions determined were as follows: pH of about 5, temperature of T=50 degrees C and enzyme to mass substrate mass ratio E/S=0.1g enzyme (g OMRS)(-1). The maximum saccharification yield obtained at optimum experimental conditions was about 50%. The experimental results agree with Lineweaver Burk's formula for low substrate concentrations. At substrate concentrations greater than 40gdm(-3), inhibitory effects were encountered. The kinetic constants obtained for the batch reactor were K(m)=0.1gdm(-3)min(-1) and V(m)=800gdm(-3).