A green fluorescent protein-based reverse two-hybrid system: application to the characterization of large numbers of potential protein-protein interactions

Methods Enzymol. 2000:328:74-88. doi: 10.1016/s0076-6879(00)28391-2.
No abstract available

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Sequence
  • DNA Primers
  • Genes, Reporter
  • Genomics
  • Green Fluorescent Proteins
  • Luminescent Proteins / biosynthesis
  • Luminescent Proteins / genetics*
  • Molecular Sequence Data
  • Mutagenesis
  • Open Reading Frames
  • Plasmids
  • Polymerase Chain Reaction / methods
  • Protein Multimerization
  • Recombinant Fusion Proteins / biosynthesis
  • Recombinant Fusion Proteins / chemistry
  • Two-Hybrid System Techniques
  • beta-Galactosidase / biosynthesis
  • beta-Galactosidase / genetics

Substances

  • DNA Primers
  • Luminescent Proteins
  • Recombinant Fusion Proteins
  • Green Fluorescent Proteins
  • beta-Galactosidase