Abstract
Single-stranded DNA was covalently bound on chip surfaces using two different silanization procedures. The resulting surfaces were characterized by fluorescence and scanning force microscopy using sequence-complementary DNA molecules with labels. Colloidal gold (30 nm) was used as the topographic label. Scanning force microscopy revealed the individual labels on the surface and their distribution. Steps of silane layers or DNA-modified surfaces prepared using an elastomeric mask provided internal controls for comparison of modified with unmodified surfaces.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Biotechnology / methods
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DNA Probes / analysis
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DNA Probes / genetics
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DNA Probes / metabolism*
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DNA, Complementary / analysis
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DNA, Complementary / genetics
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DNA, Complementary / metabolism
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Fluorescent Dyes
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Gold Colloid*
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Microscopy, Atomic Force*
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Microscopy, Fluorescence
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Oligodeoxyribonucleotides / analysis
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Oligodeoxyribonucleotides / genetics
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Oligodeoxyribonucleotides / metabolism
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Oligonucleotide Array Sequence Analysis / methods*
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Propylamines / metabolism
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Silanes / metabolism*
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Substrate Specificity
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Surface Properties
Substances
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3-glycidoxypropyltrimethoxysilane
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DNA Probes
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DNA, Complementary
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Fluorescent Dyes
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Gold Colloid
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Oligodeoxyribonucleotides
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Propylamines
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Silanes
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3-aminopropyltrimethoxysilane