VP4 and VP7 genotyping by reverse transcription-PCR of human rotavirus in mexican children with acute diarrhea

J Clin Microbiol. 2000 Oct;38(10):3876-8. doi: 10.1128/JCM.38.10.3876-3878.2000.

Abstract

Dual typing (VP4 and VP7) of rotavirus obtained from 257 Mexican children during three epidemiological seasons was performed by reverse transcription-PCR. The P1G1 genotype was the most prevalent (40%), followed by P1G3 (19%) and P2G2 (16%). Thirty-one specimens (12%) presented mixed infections, while some genotypes were not found. This is the first dual typing of isolates from diarrhea cases in Mexico.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acute Disease
  • Antigens, Viral*
  • Capsid / genetics*
  • Capsid Proteins*
  • Child
  • Diarrhea / epidemiology
  • Diarrhea / virology*
  • Genotype
  • Humans
  • Mexico / epidemiology
  • Polymerase Chain Reaction / methods
  • Reverse Transcriptase Polymerase Chain Reaction*
  • Ribotyping
  • Rotavirus / classification*
  • Rotavirus / genetics
  • Rotavirus / isolation & purification
  • Rotavirus Infections / diagnosis*
  • Rotavirus Infections / epidemiology

Substances

  • Antigens, Viral
  • Capsid Proteins
  • VP4 protein, Rotavirus
  • VP7 protein, Rotavirus