The Neurospora am gene and NADP-specific glutamate dehydrogenase: mutational sequence changes and functional effects--more mutants and a summary

J R Fincham; J A Kinsey; A M Fuentes; N J Cummings; I F Connerton

doi:10.1017/s0016672300004602

The Neurospora am gene and NADP-specific glutamate dehydrogenase: mutational sequence changes and functional effects--more mutants and a summary

Genet Res. 2000 Aug;76(1):1-10. doi: 10.1017/s0016672300004602.

Authors

J R Fincham¹, J A Kinsey, A M Fuentes, N J Cummings, I F Connerton

Affiliation

¹ Institute of Cell and Molecular Biology, University of Edinburgh, UK.

PMID: 11006629
DOI: 10.1017/s0016672300004602

Abstract

A further series of mutant am alleles, encoding potentially active NADP-specific glutamate dehydrogenase (GDH) and capable of complementation in heterocaryons, have been characterized with respect to both GDH properties and DNA sequence changes. Several mutants previously studied, and some of their same-site or second-site revertants, have also been sequenced for the first time. We present a summary of what is known of the properties of all am mutants that have been defined at the sequence level.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Amino Acids / chemistry
DNA Mutational Analysis
Genes, Fungal
Genetic Complementation Test
Genotype
Glutamate Dehydrogenase (NADP+) / chemistry
Glutamate Dehydrogenase (NADP+) / genetics*
Glutamate Dehydrogenase (NADP+) / metabolism
Glutamic Acid / pharmacology
Mutation*
Neurospora / genetics*
Nucleic Acid Conformation
Nucleotides / metabolism
Phenotype
Protein Conformation
Spectrophotometry
Time Factors

Substances

Amino Acids
Nucleotides
Glutamic Acid
Glutamate Dehydrogenase (NADP+)