SB-253514 and analogues; novel inhibitors of lipoprotein-associated phospholipase A2 produced by Pseudomonas fluorescens DSM 11579. I. Fermentation of producing strain, isolation and biological activity

J Antibiot (Tokyo). 2000 Jul;53(7):664-9. doi: 10.7164/antibiotics.53.664.

Abstract

Two new classes of inhibitors of LpPLA2 have been identified in fermentations of Pseudomonas fluorescens. The two structurally isomeric series differ in the geometry of closure of the bicyclic carbamate and comprise a range of compounds varying only in length of their lipophilic sidechain. The most abundant species were extracted from the cells and purified by silica and C18 chromatography. Members of the more stable class were shown to be potent and selective competitive inhibitors of LpPLA2.

MeSH terms

  • 1-Alkyl-2-acetylglycerophosphocholine Esterase
  • Animals
  • Bridged Bicyclo Compounds, Heterocyclic / isolation & purification*
  • Bridged Bicyclo Compounds, Heterocyclic / metabolism
  • Bridged Bicyclo Compounds, Heterocyclic / pharmacology*
  • Drug Evaluation, Preclinical / methods
  • Enzyme Inhibitors / isolation & purification*
  • Enzyme Inhibitors / metabolism
  • Enzyme Inhibitors / pharmacology*
  • Fermentation
  • Humans
  • Phospholipases A / antagonists & inhibitors*
  • Phospholipases A2
  • Pseudomonas fluorescens / metabolism*
  • Pyrans / isolation & purification*
  • Pyrans / metabolism
  • Pyrans / pharmacology*
  • Rabbits

Substances

  • Bridged Bicyclo Compounds, Heterocyclic
  • Enzyme Inhibitors
  • Pyrans
  • SB 253514
  • Phospholipases A
  • Phospholipases A2
  • 1-Alkyl-2-acetylglycerophosphocholine Esterase