Abstract
We report the engineering of Lactococcus lactis for the efficient conversion of sugar into diacetyl by combining NADH-oxidase overproduction and alpha-acetolactate decarboxylase inactivation. Eighty percent of the carbon flux was found to be rerouted via alpha-acetolactate to the production of diacetyl by preloading the cells with NADH-oxidase before their use as a cell factory.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Carboxy-Lyases / antagonists & inhibitors
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Diacetyl / metabolism*
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Genetic Engineering*
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Lactates / metabolism
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Lactococcus lactis / genetics*
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Lactococcus lactis / metabolism
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Multienzyme Complexes / genetics
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Multienzyme Complexes / metabolism
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NADH, NADPH Oxidoreductases / genetics
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NADH, NADPH Oxidoreductases / metabolism
Substances
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Lactates
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Multienzyme Complexes
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alpha-acetolactate
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NADH oxidase
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NADH, NADPH Oxidoreductases
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Carboxy-Lyases
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acetolactate decarboxylase
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Diacetyl