Fas ligand and Bax gene transcription contributes to Ro60 ribonucleoprotein redistribution in UV-A irradiated human keratinocytes

Joint Bone Spine. 2000;67(4):283-9.

Abstract

Objectives: Ro ribonucleoproteins are of particular interest because they are serologic markers of photosensitive variants of lupus such as the subacute cutaneous lupus erythematosus (SCLE), in which the polycyclic skin lesions are triggered by exposure to the sun. We study the role of apoptosis in the expression of Ro antigen.

Methods: We used UV-A irradiated keratinocytes.

Results: We demonstrate in cultured human UVA-irradiated keratinocytes that the enhanced expression of Ro60 ribonucleoprotein is caused by antigenic redistribution consecutive to Fas-L and Bax gene activation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Apoptosis / genetics*
  • Autoantigens / metabolism*
  • Autoantigens / radiation effects
  • Blotting, Western
  • Cells, Cultured
  • Fas Ligand Protein
  • Fluorescent Antibody Technique, Indirect
  • Humans
  • Image Processing, Computer-Assisted
  • In Situ Hybridization
  • Keratinocytes / metabolism
  • Keratinocytes / radiation effects*
  • Male
  • Membrane Glycoproteins / genetics*
  • Proto-Oncogene Proteins / genetics*
  • Proto-Oncogene Proteins c-bcl-2*
  • RNA, Small Cytoplasmic*
  • Ribonucleoproteins / metabolism*
  • Ribonucleoproteins / radiation effects
  • Transcription, Genetic*
  • Ultraviolet Rays
  • bcl-2-Associated X Protein

Substances

  • Autoantigens
  • BAX protein, human
  • FASLG protein, human
  • Fas Ligand Protein
  • Membrane Glycoproteins
  • Proto-Oncogene Proteins
  • Proto-Oncogene Proteins c-bcl-2
  • RNA, Small Cytoplasmic
  • RO60 protein, human
  • Ribonucleoproteins
  • bcl-2-Associated X Protein