Reversed-phase high-performance liquid chromatography combined with on-line UV diode array, FT infrared, and 1H nuclear magnetic resonance spectroscopy and time-of-flight mass spectrometry: application to a mixture of nonsteroidal antiinflammatory drugs

D Louden; A Handley; S Taylor; E Lenz; S Miller; I D Wilson; A Sage

doi:10.1021/ac000204y

Reversed-phase high-performance liquid chromatography combined with on-line UV diode array, FT infrared, and 1H nuclear magnetic resonance spectroscopy and time-of-flight mass spectrometry: application to a mixture of nonsteroidal antiinflammatory drugs

Anal Chem. 2000 Aug 15;72(16):3922-6. doi: 10.1021/ac000204y.

Authors

D Louden¹, A Handley, S Taylor, E Lenz, S Miller, I D Wilson, A Sage

Affiliation

¹ LGC, The Health, Runcorn, Cheshire, UK.

PMID: 10959983
DOI: 10.1021/ac000204y

Abstract

A prototype multiply hyphenated system has been applied to the analysis of a mixture of nonsteroidal antiinflammatory drugs separated by reversed-phase HPLC. Characterization of the model NSAIDs was achieved via a combination of diode array UV, 1H NMR, FT-IR spectroscopy, and time-of-flight mass spectrometry. This combination of spectrometers allowed the collection of UV, 1H NMR, IR, and mass spectra together with atomic composition data enabling almost complete structural characterization to be performed.

MeSH terms

Anti-Inflammatory Agents, Non-Steroidal / analysis*
Anti-Inflammatory Agents, Non-Steroidal / chemistry
Chromatography, High Pressure Liquid / methods*
Spectrum Analysis / methods*

Substances

Anti-Inflammatory Agents, Non-Steroidal