Rapid determination of vitamin E in vegetable oils by reversed-phase high-performance liquid chromatography

J Chromatogr A. 2000 Jun 9;881(1-2):251-4. doi: 10.1016/s0021-9673(00)00219-3.

Abstract

A quick and direct method for measuring tocopherols (alpha, beta+gamma and delta) in vegetable oils has been developed using RP-HPLC with UV detection. Previous extraction of tocopherols is not required. The oil is diluted in hexane and an aliquot is mixed with ethanol containing an internal standard (alpha-tocopherol acetate). The chromatographic system consists of an ODS-2 column with a methanol-water mobile phase. Tocopherols are detected at 292 nm in less than 5 min after injection. The method is precise (RSD=2.69%) and has a high mean recovery (98.14%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromatography, High Pressure Liquid / methods*
  • Plant Oils / chemistry*
  • Reproducibility of Results
  • Sensitivity and Specificity
  • Spectrophotometry, Ultraviolet
  • Vitamin E / analysis*

Substances

  • Plant Oils
  • Vitamin E