Yeast two-hybrid systems and protein interaction mapping projects for yeast and worm

Yeast. 2000 Jun 30;17(2):88-94. doi: 10.1002/1097-0061(20000630)17:2<88::AID-YEA20>3.0.CO;2-Y.

Abstract

The availability of complete genome sequences necessitates the development of standardized functional assays to analyse the tens of thousands of predicted gene products in high-throughput experimental settings. Such approaches are collectively referred to as 'functional genomics'. One approach to investigate the properties of a proteome of interest is by systematic analysis of protein-protein interactions. So far, the yeast two-hybrid system is the most commonly used method for large-scale, high-throughput identification of potential protein-protein interactions. Here, we discuss several technical features of variants of the two-hybrid systems in light of data recently obtained from different protein interaction mapping projects for the budding yeast Saccharomyces cerevisiae and the nematode Caenorhabditis elegans.

Publication types

  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Animals
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism
  • Fungal Proteins / genetics
  • Fungal Proteins / metabolism*
  • Helminth Proteins / genetics
  • Helminth Proteins / metabolism*
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae / metabolism
  • Two-Hybrid System Techniques*

Substances

  • Fungal Proteins
  • Helminth Proteins