Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAF(I)48, TAF(I)63 and TAF(I)110 subunits of class I general transcription initiation factor SL1

C Di Pietro; A Rapisarda; V Amico; C Bonaiuto; A Viola; M Scalia; S Motta; A Amato; H Engel; A Messina; G Sichel; K Grzeschik; M Purrello

doi:10.1159/000015592

Genomic localization of the human genes TAF1A, TAF1B and TAF1C, encoding TAF(I)48, TAF(I)63 and TAF(I)110 subunits of class I general transcription initiation factor SL1

Cytogenet Cell Genet. 2000;89(1-2):133-6. doi: 10.1159/000015592.

Authors

C Di Pietro¹, A Rapisarda, V Amico, C Bonaiuto, A Viola, M Scalia, S Motta, A Amato, H Engel, A Messina, G Sichel, K Grzeschik, M Purrello

Affiliation

¹ Dipartimento di Scienze biomediche, Sezione di Biologia generale, cellulare e di Genetica molecolare, Università di Catania, Catania, Italy.

PMID: 10894955
DOI: 10.1159/000015592

Abstract

Human SL1 is a general transcription initiation factor (GTF) essential for RNA polymerase I to start rRNA synthesis at class I promoters. It is comprised of the TATA box-binding protein (TBP) and three TBP-associated factors (TAF(I)48, TAF(I)63 and TAF(I)110). We have determined that the human genes TAF1A, TAF1B and TAF1C, encoding these three TAF(I) polypeptides, are localized at lq42, 2p25 and 16q24, respectively. All three genes are present as single copies in the human genome and map to different chromosomes, as shown by somatic cell hybrid panel and radiation hybrid panel analysis and FISH. Two of these genes, TAF1C and TAF1B, are transcribed into multiple RNAs, as determined through Northern analysis of mRNA from various human organs and cell lines. If translated into different polypeptides, this could result in production of variant isoforms of SL1 with different activation potentials.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Chromosomes, Human, Pair 1 / genetics
Chromosomes, Human, Pair 16 / genetics
Chromosomes, Human, Pair 2 / genetics
DNA-Binding Proteins / chemistry
DNA-Binding Proteins / genetics*
Genetic Linkage / genetics
Humans
Hybrid Cells
In Situ Hybridization, Fluorescence
Physical Chromosome Mapping*
Pol1 Transcription Initiation Complex Proteins*
Polymorphism, Restriction Fragment Length
Protein Isoforms / genetics
RNA, Messenger / analysis
RNA, Messenger / genetics
TATA-Binding Protein Associated Factors*
Transcription Factor TFIID*
Transcription Factors / chemistry
Transcription Factors / genetics*
Tumor Cells, Cultured

Substances

DNA-Binding Proteins
Pol1 Transcription Initiation Complex Proteins
Protein Isoforms
RNA, Messenger
TAF1A protein, human
TAF1B protein, human
TAF1C protein, human
TATA-Binding Protein Associated Factors
Transcription Factor TFIID
Transcription Factors
transcription initiation factor TIF-IB