Screening of environmental DNA libraries for the presence of genes conferring lipolytic activity on Escherichia coli

Appl Environ Microbiol. 2000 Jul;66(7):3113-6. doi: 10.1128/AEM.66.7.3113-3116.2000.

Abstract

Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Electrophoresis, Polyacrylamide Gel
  • Escherichia coli / enzymology*
  • Escherichia coli / genetics
  • Esterases / genetics*
  • Esterases / metabolism
  • Gene Library*
  • Lipase / genetics*
  • Lipase / metabolism
  • Molecular Sequence Data
  • Plasmids / genetics
  • Soil Microbiology*
  • Triglycerides / metabolism
  • Triolein / metabolism

Substances

  • Triglycerides
  • Triolein
  • Esterases
  • Lipase
  • tributyrin

Associated data

  • GENBANK/AF223645
  • GENBANK/AF223646
  • GENBANK/AF223647
  • GENBANK/AF223648