Abstract
Environmental DNA libraries prepared from three different soil samples were screened for genes conferring lipolytic activity on Escherichia coli clones. Screening on triolein agar revealed 1 positive clone out of 730,000 clones, and screening on tributyrin agar revealed 3 positive clones out of 286,000 E. coli clones. Substrate specificity analysis revealed that one recombinant strain harbored a lipase and the other three contained esterases. The genes responsible for the lipolytic activity were identified and characterized.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Electrophoresis, Polyacrylamide Gel
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Escherichia coli / enzymology*
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Escherichia coli / genetics
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Esterases / genetics*
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Esterases / metabolism
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Gene Library*
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Lipase / genetics*
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Lipase / metabolism
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Molecular Sequence Data
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Plasmids / genetics
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Soil Microbiology*
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Triglycerides / metabolism
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Triolein / metabolism
Substances
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Triglycerides
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Triolein
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Esterases
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Lipase
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tributyrin
Associated data
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GENBANK/AF223645
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GENBANK/AF223646
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GENBANK/AF223647
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GENBANK/AF223648