Facilitated tip-positioning and applications of non-electrode tips in scanning electrochemical microscopy using a shear force based constant-distance mode

Chemistry. 2000 May 2;6(9):1547-54. doi: 10.1002/(sici)1521-3765(20000502)6:9<1547::aid-chem1547>3.3.co;2-3.

Abstract

In scanning electrochemical microscopy (SECM) a microelectrode is usually scanned over a sample without following topographic changes (constant-height mode). Therefore, deconvolution of effects from distance variations arising from non-flat sample surface and electrochemical surface properties is in general not possible. Using a shear force-based constant distance mode, information about the morphology of a sample and its localized electrochemical activity can be obtained simultaneously. The setup of the SECM with integrated constant-distance mode and its application to non-flat or tilted surfaces, as well as samples with three-dimensional surface structures are presented and discussed. The facilitated use of non-amperometric tips in SECM like enzyme-filled glass capillaries is demonstrated.

MeSH terms

  • Electrochemistry
  • Glucose 1-Dehydrogenase
  • Glucose Dehydrogenases / chemistry
  • Glucose Oxidase / chemistry
  • Lasers
  • Microscopy, Electron / instrumentation
  • Microscopy, Electron / methods*
  • NAD / chemistry
  • Oxidation-Reduction

Substances

  • NAD
  • Glucose Dehydrogenases
  • Glucose 1-Dehydrogenase
  • Glucose Oxidase