We have measured the diffuse scattering in magnetite as a function of temperature using the LEO 912Ohms energy-filtering electron microscope and the imaging plates. This study takes the advantage of the Koehler illumination system, energy filtering and the imaging plates for recording electron diffraction pattern over a large dynamic range. The experiment clearly shows a quantitative change in diffuse scattering distribution, which has the characteristics of one-dimensional ordering. This study clearly demonstrates the possibility for the quantitative study of diffuse scattering using electron diffraction.