A basic 18-amino acid peptide contains the polysulfate-binding domain responsible for activation of the boar proacrosin/acrosin system

Biol Reprod. 2000 Jun;62(6):1536-42. doi: 10.1095/biolreprod62.6.1536.

Abstract

Proacrosin is the zymogen of acrosin, a serine protease localized in the acrosomal matrix of mammalian sperm. Proacrosin/acrosin binds to solubilized zona pellucida glycoproteins (ZPGs) and various polysulfates in a non-enzymatic mechanism. In addition, both polysulfates and ZPGs induce proacrosin activation once they bind to the polysulfate-binding domain (PSBD) of the enzyme. We show here that the peptide (43)IFMYHNNRRYHTCGGILL(60) inhibited the proacrosin activation induced by either fucoidan or ZPGs. In addition, the peptide was recognized by the monoclonal antibody C5F10, which is directed against the PSBD region. Our data suggest that the PSBD is composed of many "subsites" that may or may not interact with each other.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acrosin / antagonists & inhibitors
  • Acrosin / chemistry*
  • Acrosin / metabolism*
  • Amino Acid Sequence
  • Animals
  • Antibodies, Monoclonal / immunology
  • Antibody Specificity
  • Binding Sites
  • Egg Proteins / metabolism
  • Egg Proteins / pharmacology
  • Enzyme Activation / drug effects
  • Enzyme Precursors / chemistry*
  • Enzyme Precursors / metabolism*
  • Epitopes / immunology
  • Male
  • Membrane Glycoproteins / metabolism
  • Membrane Glycoproteins / pharmacology
  • Molecular Sequence Data
  • Peptide Fragments / chemistry*
  • Peptide Fragments / pharmacology
  • Polysaccharides / pharmacology
  • Receptors, Cell Surface*
  • Sulfates / metabolism*
  • Swine
  • Zona Pellucida Glycoproteins

Substances

  • Antibodies, Monoclonal
  • Egg Proteins
  • Enzyme Precursors
  • Epitopes
  • Membrane Glycoproteins
  • Peptide Fragments
  • Polysaccharides
  • Receptors, Cell Surface
  • Sulfates
  • Zona Pellucida Glycoproteins
  • fucoidan
  • proacrosin
  • Acrosin