Abstract
The complete nucleotide sequence of Tn10 has been determined. The dinucleotide signature and percent G+C of the sequence had no discontinuities, indicating that Tn10 constitutes a homogeneous unit. The new sequence contained three new open reading frames corresponding to a glutamate permease, repressors of heavy metal resistance operons, and a hypothetical protein in Bacillus subtilis. The glutamate permease was fully functional when expressed, but Tn10 did not protect Escherichia coli from the toxic effects of various metals.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Transport Systems, Acidic*
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Base Sequence
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DNA Transposable Elements*
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DNA, Bacterial*
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Enterobacter cloacae / genetics
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Escherichia coli Proteins
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Membrane Transport Proteins / genetics
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Molecular Sequence Data
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Salmonella / genetics
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Shigella flexneri / genetics*
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Sodium / metabolism
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Symporters
Substances
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Amino Acid Transport Systems, Acidic
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DNA Transposable Elements
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DNA, Bacterial
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Escherichia coli Proteins
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Membrane Transport Proteins
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Symporters
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gltS protein, E coli
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Sodium