Abstract
Extracellular enzymes--a chitinase and a protease with molecular weights 22 and 32 kDa, respectively--were isolated from Streptomyces kurssanovii cells. After purification on modified regenerated chitin, the enzymes were virtually homogeneous according to denaturing PAGE. Both enzymes were found to degrade chitosan.
MeSH terms
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Chitin / metabolism
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Chitinases / biosynthesis
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Chitinases / isolation & purification*
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Chitinases / metabolism
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Electrophoresis, Polyacrylamide Gel
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Endopeptidases / biosynthesis
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Endopeptidases / isolation & purification*
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Endopeptidases / metabolism
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Extracellular Space / enzymology
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Hydrolysis
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Molecular Weight
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Streptomyces / enzymology*
Substances
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Chitin
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Chitinases
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Endopeptidases