Use of the two-hybrid system to identify Rab-interacting proteins

I Janoueix-Lerosey; B Goud

doi:10.1006/meth.2000.0952

Use of the two-hybrid system to identify Rab-interacting proteins

Methods. 2000 Apr;20(4):399-402. doi: 10.1006/meth.2000.0952.

Authors

I Janoueix-Lerosey¹, B Goud

Affiliation

¹ INSERM U 509, Laboratoire Mécanismes moléculaires du transport intracellulaire, UMR CNRS 144, Institut Curie, 26 rue d'Ulm, Paris Cedex 05, 75248, France.

PMID: 10720460
DOI: 10.1006/meth.2000.0952

Abstract

The yeast two-hybrid system has been useful for identifying many partners and effectors of small GTPases of the Rab family. We describe here such a screen using Rab6, a protein involved in the regulation of intracellular transport at the level of the Golgi apparatus, as bait.

MeSH terms

Guanine Nucleotide Dissociation Inhibitors / genetics
Guanine Nucleotide Dissociation Inhibitors / isolation & purification*
Protein Binding
Protein Isoforms / genetics
Protein Isoforms / isolation & purification
Two-Hybrid System Techniques*
rab GTP-Binding Proteins / metabolism*
rho-Specific Guanine Nucleotide Dissociation Inhibitors

Substances

Guanine Nucleotide Dissociation Inhibitors
Protein Isoforms
Rab6 protein
rho-Specific Guanine Nucleotide Dissociation Inhibitors
rab GTP-Binding Proteins