Suppression of GD1alpha ganglioside-mediated tumor metastasis by liposomalized WHW-peptide

FEBS Lett. 2000 Jan 28;466(2-3):381-4. doi: 10.1016/s0014-5793(00)01110-8.

Abstract

GD1alpha ganglioside-replica peptides were recently isolated from a phage-displayed random pentadecapeptide library by assaying for inhibition of adhesion of RAW117-H10 lymphosarcoma cells to hepatic sinusoidal microvessel endothelial (HSE) cells. We show here that the Trp-His-Trp (WHW) peptide was identified as a minimal sequence of the GD1alpha-replica peptide WHWRHRIPLQLAAGR. The addition of WHW peptide-attached liposomes displayed efficient inhibition of liver metastasis of RAW117-H10 cells as well as of GD1alpha-mediated adhesion of RAW117-H10 cells to HSE cells in vitro. These results suggest that engineered liposomes for peptide delivery are applicable to treatment for metastasis.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Cell Adhesion / drug effects
  • Drug Carriers
  • G(M1) Ganglioside / analogs & derivatives*
  • G(M1) Ganglioside / antagonists & inhibitors
  • Liposomes
  • Liver Neoplasms, Experimental / drug therapy*
  • Liver Neoplasms, Experimental / secondary
  • Mice
  • Mice, Inbred BALB C
  • Molecular Sequence Data
  • Neoplasm Metastasis
  • Peptides / pharmacology*
  • Proteins / pharmacology*
  • Tumor Cells, Cultured

Substances

  • Drug Carriers
  • GD1alpha-replica peptide
  • Liposomes
  • Peptides
  • Proteins
  • ganglioside GD1alpha
  • G(M1) Ganglioside