Arabidopsis cytochrome P450s that catalyze the first step of tryptophan-dependent indole-3-acetic acid biosynthesis

Proc Natl Acad Sci U S A. 2000 Feb 29;97(5):2379-84. doi: 10.1073/pnas.040569997.

Abstract

Plants synthesize numerous secondary metabolites that are used as developmental signals or as defense against pathogens. Tryptophan (Trp)-derived secondary metabolites include camalexin, indole glucosinolates, and indole-3-acetic acid (IAA); however, the steps in their synthesis from Trp or its precursors remain unclear. We have identified two Arabidopsis cytochrome P450s (CYP79B2 and CYP79B3) that can convert Trp to indole-3-acetaldoxime (IAOx), a precursor to IAA and indole glucosinolates.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Arabidopsis / genetics
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / isolation & purification
  • Arabidopsis Proteins / metabolism*
  • Catalysis
  • Cytochrome P-450 Enzyme System / genetics
  • Cytochrome P-450 Enzyme System / isolation & purification
  • Cytochrome P-450 Enzyme System / metabolism*
  • Genes, Plant
  • Indoleacetic Acids / metabolism*
  • Molecular Sequence Data
  • Oximes / metabolism
  • Plants, Genetically Modified
  • Pseudomonas / physiology
  • Tryptophan / metabolism*

Substances

  • Arabidopsis Proteins
  • Indoleacetic Acids
  • Oximes
  • indoleacetic acid
  • Tryptophan
  • Cytochrome P-450 Enzyme System
  • cytochrome P-450 79B3, Arabidopsis
  • acetaldehyde oxime

Associated data

  • GENBANK/UNKNOWN