Neutralization of the hemorrhagic activity of Bothrops and Lachesis snake venoms by a monoclonal antibody against mutalysin-II

Toxicon. 2000 Jan;38(1):139-44. doi: 10.1016/s0041-0101(99)00134-8.

Abstract

One mAb reactive with mutalysin-II, a hemorrhagic metalloproteinase isolated from Lachesis muta muta venom, was produced in mice immunized with L. m. muta venom. Indirect ELISA was employed to compare the antigenic cross-reactivity among the venoms from Bothrops snakes. The mAb anti-mutalysin-II efficiently neutralized the hemorrhagic effect of both mutalysin-II and L. m. muta crude venom. Furthermore, the mAb were cross-reactive with B. alternatus, B. atrox, B. itapetiningae, B. jararaca and B. neuwiedii and showed variable potencies in neutralizing the hemorrhagic activity of several bothropic venoms.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / pharmacology*
  • Bothrops*
  • Cell Fusion / drug effects
  • Cross Reactions
  • Crotalid Venoms / antagonists & inhibitors*
  • Crotalid Venoms / toxicity*
  • Electrophoresis, Polyacrylamide Gel
  • Enzyme-Linked Immunosorbent Assay
  • Hemorrhage / chemically induced*
  • Hemorrhage / prevention & control*
  • Male
  • Metalloendopeptidases / antagonists & inhibitors*
  • Metalloendopeptidases / toxicity
  • Mice
  • Mice, Inbred BALB C
  • Spleen / cytology
  • Viper Venoms / antagonists & inhibitors*
  • Viper Venoms / toxicity*
  • Viperidae*

Substances

  • Antibodies, Monoclonal
  • Crotalid Venoms
  • Viper Venoms
  • Metalloendopeptidases
  • hemorrhagic factor LHFII