Native plasmids of Fusobacterium nucleatum: characterization and use in development of genetic systems

J Bacteriol. 2000 Feb;182(4):1176-80. doi: 10.1128/JB.182.4.1176-1180.2000.

Abstract

Three native plasmids of Fusobacterium nucleatum were characterized, including DNA sequence analysis of one plasmid, pFN1. A shuttle plasmid, pHS17, capable of transforming Escherichia coli and F. nucleatum ATCC 10953 was constructed with pFN1. pHS17 was stably maintained in the F. nucleatum transformants, and differences in the transformation efficiencies suggested the presence of a restriction-modification system in F. nucleatum.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • DNA Nucleotidyltransferases*
  • DNA, Bacterial / analysis
  • Endodeoxyribonucleases / chemistry
  • Escherichia coli / genetics
  • Fusobacterium nucleatum / enzymology
  • Fusobacterium nucleatum / genetics*
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Sequence Analysis, DNA
  • Transformation, Bacterial*

Substances

  • DNA, Bacterial
  • DNA Nucleotidyltransferases
  • DNA relaxase
  • Endodeoxyribonucleases

Associated data

  • GENBANK/AF159249
  • GENBANK/AF219231