Isolation and structure elucidation of new cytotoxic steroids from the gorgonian Leptogorgia sarmentosa

Steroids. 2000 Feb;65(2):85-8. doi: 10.1016/s0039-128x(99)00083-5.

Abstract

The gorgonian Leptogorgia sarmentosa contains three new steroids, (20S)-20-hydroxycholestane-3,16-dione (1), (16S, 20S)-16,20-dihydroxycholestan-3-one (2), and (20S)-20-hydroxycholest-1-ene-3,16-dione (3) together with a known related compound (4). Their structures were defined by spectroscopic analysis. The new steroids exhibited significant cytotoxicity against four tumor cell lines (ED50 = 1 microg/ml).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Survival
  • Cholestanones / chemistry*
  • Cholestanones / isolation & purification*
  • Cholestanones / toxicity
  • Cnidaria / chemistry*
  • Cytotoxins / chemistry*
  • Cytotoxins / isolation & purification
  • Humans
  • Mice
  • Molecular Structure
  • Nuclear Magnetic Resonance, Biomolecular
  • Spain
  • Steroids / chemistry*
  • Steroids / isolation & purification*
  • Steroids / toxicity
  • Tumor Cells, Cultured

Substances

  • 16,20-dihydroxycholestan-3-one
  • 20-hydroxycholest-1-en-3,16-dione
  • 20-hydroxycholestane-3,16-dione
  • Cholestanones
  • Cytotoxins
  • Steroids