Abstract
A novel isoform of a major rye grass pollen allergen Lol p 5 was isolated from a cDNA expression library. The new isoform, Lol p 5C, shares 95% amino acid sequence identity with Lol p 5A. Both isoforms demonstrated shared antigenic activity but different allergenic activities. Recombinant Lol p 5C demonstrated 100% IgE reactivity in 22 rye grass pollen sensitive patients. In comparison, recombinant Lol p 5A showed IgE reactivity in less than 64% of the patients. Therefore, Lol p 5C represents a novel and highly IgE-reactive isoform allergen of rye grass pollen.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Allergens*
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Amino Acid Sequence
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Antibodies, Monoclonal / metabolism
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Antigens, Plant
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Base Sequence
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Cloning, Molecular
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DNA, Complementary / metabolism
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Electrophoresis, Gel, Two-Dimensional
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Gene Library
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Humans
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Hypersensitivity / immunology
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Immunoblotting
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Immunoglobulin E / metabolism*
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Lolium / genetics
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Molecular Sequence Data
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Plant Proteins / biosynthesis*
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Plant Proteins / genetics*
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Plant Proteins / immunology
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Protein Isoforms / biosynthesis
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Protein Isoforms / genetics
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Protein Isoforms / immunology
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Sequence Homology, Amino Acid
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Time Factors
Substances
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Allergens
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Antibodies, Monoclonal
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Antigens, Plant
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DNA, Complementary
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Lol p V protein, Lolium perenne
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Plant Proteins
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Protein Isoforms
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Immunoglobulin E