Disulfide cross-linking of subunits F(1)-gamma and F(0)I-PVP(b) results in asymmetric effects on proton translocation in the mitochondrial ATP synthase

FEBS Lett. 1999 Dec 10;463(1-2):7-11. doi: 10.1016/s0014-5793(99)01593-8.

Abstract

A study is presented on the effect of diamide-induced disulfide cross-linking of F(1)-gamma and F(0)I-PVP(b) subunits on proton translocation in the mitochondrial ATP synthase. The results show that, upon cross-linking of these subunits, whilst proton translocation from the A side to the B F(1) side is markedly accelerated with decoupling of oxidative phosphorylation, proton translocation in the reverse direction, driven by either ATP hydrolysis or a diffusion potential, is unaffected. These observations reveal further peculiarities of the mechanism of energy transfer in the ATP synthase of coupling membranes.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biological Transport / drug effects
  • Cattle
  • Cross-Linking Reagents / pharmacology
  • Diamide / pharmacology
  • Disulfides / chemistry*
  • Kinetics
  • Mitochondria / enzymology*
  • Oligomycins / pharmacology
  • Proton-Translocating ATPases / chemistry*
  • Proton-Translocating ATPases / metabolism*
  • Protons*
  • Sulfhydryl Reagents / metabolism
  • Uncoupling Agents / pharmacology

Substances

  • Cross-Linking Reagents
  • Disulfides
  • Oligomycins
  • Protons
  • Sulfhydryl Reagents
  • Uncoupling Agents
  • Diamide
  • Proton-Translocating ATPases