Abstract
Adeno-associated virus (AAV) is inefficient at infecting differentiated airway epithelia because of a lack of receptors at the apical surface. We hypothesized that incorporation of AAV in a calcium phosphate coprecipitate would circumvent this barrier. Interestingly, coprecipitation of AAV type 2 improved gene transfer to differentiated human airway epithelia in vitro and to the mouse lung in vivo. These results suggest that delivery of AAV as a CaP(i) coprecipitate may significantly enhance its utility for gene transfer to the airway epithelia in vivo.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Bronchi / metabolism*
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Calcium Phosphates / chemistry*
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Chemical Precipitation
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Dependovirus / chemistry
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Dependovirus / genetics*
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Epithelium / metabolism
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Gene Transfer Techniques / standards*
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Humans
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In Vitro Techniques
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Mice
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Mice, Inbred C57BL
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Recombination, Genetic
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Trachea / metabolism*
Substances
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Calcium Phosphates
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alpha-tricalcium phosphate
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tetracalcium phosphate
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calcium phosphate, monobasic, anhydrous
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calcium phosphate
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calcium phosphate, dibasic, anhydrous