Preparation of chitooligosaccharides from chitosan by a complex enzyme

H Zhang; Y Du; X Yu; M Mitsutomi; S Aiba

doi:10.1016/s0008-6215(99)00154-8

Preparation of chitooligosaccharides from chitosan by a complex enzyme

Carbohydr Res. 1999 Aug 15;320(3-4):257-60. doi: 10.1016/s0008-6215(99)00154-8.

Authors

H Zhang¹, Y Du, X Yu, M Mitsutomi, S Aiba

Affiliation

¹ Department of Biochemical Engineering, Dalian Institute of Chemical Physics, Chinese Academy of Science, People's Republic of China.

PMID: 10573862
DOI: 10.1016/s0008-6215(99)00154-8

Abstract

Chitosan of 24% degree of acetylation was depolymerized by a mixture of cellulase, alpha amylase, and proteinase to give the title oligosaccharides. The removal of products by membrane separation permitted yield maximization of products having degree of polymerization in the 3-10 range.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chitin / analogs & derivatives*
Chitin / chemistry
Chitin / metabolism
Chitosan
Enzymes / metabolism
Mass Spectrometry
Oligosaccharides / biosynthesis*

Substances

Enzymes
Oligosaccharides
Chitin
Chitosan