Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1

M J Fernández; J L Adrio; J M Piret; S Wolfe; S Ro; A L Demain

doi:10.1007/s002530051549

Stimulatory effect of growth in the presence of alcohols on biotransformation of penicillin G into cephalosporin-type antibiotics by resting cells of Streptomyces clavuligerus NP1

Appl Microbiol Biotechnol. 1999 Oct;52(4):484-8. doi: 10.1007/s002530051549.

Authors

M J Fernández¹, J L Adrio, J M Piret, S Wolfe, S Ro, A L Demain

Affiliation

¹ Department of Biology, Massachusetts Institute of Technology, Cambridge 02139, USA.

PMID: 10570794
DOI: 10.1007/s002530051549

Abstract

Growth of Streptomyces clavuligerus NP1 in the presence of methanol or ethanol resulted in a marked increase in production of cephalosporin(s) from penicillin G by resting cells. The mycelium produced in alcohol-supplemented medium was fragmented and dispersed as compared with growth in control medium. HPLC analysis showed that at least two products were present in the biotransformation supernatant fluid after 1 h incubation. One of them has been identified as deacetoxycephalosporin G (DAOG).

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alcohols / pharmacology*
Biotransformation / drug effects
Cephalosporins / analysis
Cephalosporins / metabolism*
Chromatography, High Pressure Liquid
Culture Media / chemistry
Ethanol / pharmacology
Methanol / pharmacology
Penicillin G / metabolism*
Penicillins / metabolism*
Streptomyces / drug effects*
Streptomyces / growth & development
Streptomyces / metabolism
Time Factors

Substances

Alcohols
Cephalosporins
Culture Media
Penicillins
Ethanol
Penicillin G
Methanol