Time-resolved fluoroimmunoassay for delta(9)-tetrahydrocannabinol as applied to early discrimination of Cannabis sativa plants

M A Bacigalupo; A Ius; G Meroni; G Grassi; A Moschella

doi:10.1021/jf981141b

Time-resolved fluoroimmunoassay for delta(9)-tetrahydrocannabinol as applied to early discrimination of Cannabis sativa plants

J Agric Food Chem. 1999 Jul;47(7):2743-5. doi: 10.1021/jf981141b.

Authors

M A Bacigalupo¹, A Ius, G Meroni, G Grassi, A Moschella

Affiliation

¹ Istituto di Biocatalisi e Riconoscimento Molecolare, CNR, Via Mario Bianco 9, Milano 20131, Italy. bacigalupo@ico.mi.cnr.it

PMID: 10552557
DOI: 10.1021/jf981141b

Abstract

In the cultivations of Cannabis, it is important to be able to distinguish fiber-type plants from drug-type plants by an easy observation of their phenotype. This study required the screening of many samples for their cannabinoid content. A simple and highly sensitive time-resolved fluoroimmunological method was developed for the determination of Delta(9)-tetrahydrocannabinol in the leaf extracts. The useful range of the calibration curve was between 10 pg and 25 ng of standard. Matrix effects were minimized by a high dilution of samples.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cannabis / classification*
Chromatography, Gas
Dronabinol / analysis*
Fluoroimmunoassay / methods*
Mice
Mice, Inbred BALB C

Substances

Dronabinol