Effect of tyrosinase inhibitors on Tuber borchii mycelium growth in vitro

FEMS Microbiol Lett. 1999 Nov 1;180(1):69-75. doi: 10.1111/j.1574-6968.1999.tb08779.x.

Abstract

This paper reports the effect of the tyrosinase (monophenol o-diphenol:oxygen oxidoreductase; EC 1.14.18.1) inhibitors diethyldithiocarbamate (DETC), L-tropolone, kojic acid, phenylthiourea (PTU) and L-mimosine on the in vitro growth of Tuber borchii (a white truffle) mycelium. A significant inhibitory effect on mycelium growth was observed for DETC, PTU and L-tropolone (0% growth compared to control at 100 microg ml(-1) DETC, PTU and L-tropolone and at 10 microg ml(-1) DETC and L-tropolone). As a comparison the action of the same inhibitors was also tested on the growth and pigmentation of the mould Cladosporium sphaerospermum. In the presence of CuSO(4) 10(-6) M T. borchii mycelium acquired pigmentation (as rounded aggregates compared to control revealed by SEM microscopy). Tyrosinase activity in the extract from T. borchii mycelium (18-day culture) was detected spectrophotometrically.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Ascomycota / drug effects*
  • Ascomycota / growth & development
  • Cladosporium / growth & development
  • Copper Sulfate
  • Culture Media / chemistry
  • Ditiocarb / pharmacology
  • Enzyme Inhibitors / pharmacology*
  • Microscopy, Electron, Scanning
  • Mimosine / pharmacology
  • Monophenol Monooxygenase / antagonists & inhibitors*
  • Monophenol Monooxygenase / metabolism
  • Mycotoxins / pharmacology
  • Phenylthiourea / pharmacology
  • Pigments, Biological
  • Pyrones / pharmacology
  • Tropolone / pharmacology

Substances

  • Culture Media
  • Enzyme Inhibitors
  • Mycotoxins
  • Pigments, Biological
  • Pyrones
  • Mimosine
  • Phenylthiourea
  • kojic acid
  • Tropolone
  • Ditiocarb
  • Monophenol Monooxygenase
  • Copper Sulfate