Protection of MLV vector particles from human complement

S Breun; B Salmons; W H Günzburg; J G Baumann

doi:10.1006/bbrc.1999.1474

Protection of MLV vector particles from human complement

Biochem Biophys Res Commun. 1999 Oct 14;264(1):1-5. doi: 10.1006/bbrc.1999.1474.

Authors

S Breun¹, B Salmons, W H Günzburg, J G Baumann

Affiliation

¹ Institute of Virology, University of Veterinary Sciences, Veterinärplatz 1, Vienna, A-1210, Austria.

PMID: 10527830
DOI: 10.1006/bbrc.1999.1474

Abstract

Murine cell-derived MLV vector particles usually are highly sensitive to human complement-mediated lysis. Expression of the human complement inhibitor CD59 on murine packaging cells resulted in partial protection of these cells from lysis caused by human complement proteins. Furthermore, CD59 was incorporated into MLV vector particles released by these packaging cells, leading to an improved resistance of the virions against human complement-mediated inactivation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
CD59 Antigens / genetics
CD59 Antigens / immunology*
Cells, Cultured
Complement Inactivator Proteins / genetics
Complement Inactivator Proteins / immunology*
Complement System Proteins / immunology*
Complement System Proteins / metabolism
Genetic Vectors / genetics*
Humans
Leukemia Virus, Murine / genetics
Leukemia Virus, Murine / immunology*
Mice
Transfection

Substances

CD59 Antigens
Complement Inactivator Proteins
Complement System Proteins