Nineteen isolates of Staphylococcus epidermidis from patients with ocular infections were analyzed. Patients were selected in retrospect, by choosing cases in which S. epidermidis was the sole isolate. Twelve different patterns were obtained after hybridization with a probe with high-level homology to insertion sequences found in S. epidermidis. Susceptibilities to penicillin, methicillin, gentamicin, tetracycline, erythromycin, ciprofloxacin, vancomycin, and teicoplanin were determined. Six strains were resistant to three or more antibiotics.