Abstract
Intranasal infection of mice by Vesicular Stomatitis Virus (VSV) often leads to breakdown of the blood-brain barrier (BBB). The role of Interleukin 12 (IL-12) and nitric oxide synthase (NOS) was examined here. Wild-type (WT), NOS-1 knockout (KO), and NOS-3 KO mice were infected with VSV and treated with either IL-12 or medium. IL-12 treatment of uninfected hosts did not result in pathology. In contrast with WT and NOS-1 KO mice, where extensive gross and ultrastructural correlation of BBB breakdown were evident following infection, in NOS-3 KO mice, integrity of the BBB was observed. Thus NOS-3 activity in astrocytes, endothelial cells, or ependymal cells may play an essential role in regulating the BBB.
Copyright 1999 Academic Press.
Publication types
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Comparative Study
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Astrocytes / enzymology
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Blood-Brain Barrier / physiology*
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Brain / blood supply
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Brain / ultrastructure
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Coloring Agents
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Encephalitis, Viral / enzymology
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Encephalitis, Viral / pathology
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Encephalitis, Viral / physiopathology*
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Endothelium, Vascular / enzymology
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Evans Blue
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Gap Junctions / ultrastructure
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Immunity, Innate
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Interleukin-12 / physiology*
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Male
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Mice
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Mice, Inbred C57BL
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Mice, Knockout
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Nitric Oxide / physiology*
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Nitric Oxide Synthase / deficiency
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Nitric Oxide Synthase / genetics
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Nitric Oxide Synthase / physiology*
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Nitric Oxide Synthase Type II
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Nitric Oxide Synthase Type III
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Rhabdoviridae Infections / enzymology
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Rhabdoviridae Infections / pathology
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Rhabdoviridae Infections / physiopathology*
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Specific Pathogen-Free Organisms
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Vesicular stomatitis Indiana virus*
Substances
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Coloring Agents
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Interleukin-12
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Nitric Oxide
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Evans Blue
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Nitric Oxide Synthase
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Nitric Oxide Synthase Type II
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Nitric Oxide Synthase Type III
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Nos2 protein, mouse
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Nos3 protein, mouse