Synthesis of a new analog of thymidine for in vivo non-radioactive labeling of DNA

C Rodríguez-Tanty; R Pérez; J Miranda; H Vélez-Castro; A Rosado; A Macías; L Galán; D Higginson-Clarke; A M Riverón

doi:10.1080/15257779908041663

Synthesis of a new analog of thymidine for in vivo non-radioactive labeling of DNA

Nucleosides Nucleotides. 1999 Apr-May;18(4-5):1113-7. doi: 10.1080/15257779908041663.

Authors

C Rodríguez-Tanty¹, R Pérez, J Miranda, H Vélez-Castro, A Rosado, A Macías, L Galán, D Higginson-Clarke, A M Riverón

Affiliation

¹ Organic Chemistry Department, National Center for Scientific Research, Havana, Cuba.

PMID: 10432745
DOI: 10.1080/15257779908041663

Abstract

The introduction of 6-(p-bromobenzoylamino)caproyl radical in the methyl group of 2'-O-deoxythymidine is described. In vivo incorporation of this nucleoside to DNA was determined using a monoclonal antibody that recognized the radical.

MeSH terms

DNA, Bacterial / chemistry*
Escherichia coli / genetics
Magnetic Resonance Spectroscopy
Spectrometry, Mass, Fast Atom Bombardment
Spectrophotometry, Infrared
Thymidine / analogs & derivatives*
Thymidine / chemical synthesis

Substances

DNA, Bacterial
Thymidine