[An investigation of the polymorphism of HLA class II alleles in the Han population in Hubei Province of China]

F Gong; P Xiong; Z Yang; Y Xu; X Jiang; X Wu; M Liu; W Fong; S Ferencik; J Liu; H Grosse-Wilde

[An investigation of the polymorphism of HLA class II alleles in the Han population in Hubei Province of China]

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 1999 Aug;16(4):216-9.

[Article in Chinese]

Authors

F Gong¹, P Xiong, Z Yang, Y Xu, X Jiang, X Wu, M Liu, W Fong, S Ferencik, J Liu, H Grosse-Wilde

Affiliation

¹ Department of Immunology, Tongji Medical University, Wuhan, 430030 P. R.China. myxjys@tjmu.edu.cn

PMID: 10431045

Abstract

Objective: To investigate the polymorphism of HLA class II alleles in the Han population in Hubei province.

Methods: The alleles of DRB1*(n=168), DQB1*(n=160) and DPB1*(n=93) were typed by using the polymerase chain reaction/sequence specific primer(PCR/SSP) and polymerase chain reaction/restriction fragment length polymorphism (PCR/RFLP) techniques.

Results: 39 HLA-DRB1, 15 DQB1 and 17 DPB1 alleles were found. Alleles with higher frequencies are DRB1*0901 (GF=14%), *1501(GF=11.3%), *0301(GF=7.1%), *0803(GF=4.8%); DQB1*0301(GF=18.8%), *0303(GF=18.4%), *0201(GF=10%), *0302(GF=8.4%); DPB1*0501(GF=31.2%), *0401(GF=15.1%), *0201(GF=14%), *0402(GF=11.8%), respectively. As compared with Caucasians, the Han people in Hubei province have higher gene frequencies of DRB1*0901, *1001, *0803; DQB1*0303, *0502 and DPB1*0501, *0202; and lower gene frequencies of DRB1*0301, *0401, *1301; DQB1*0201, *0603 and DPB1*0401, suggesting that Hubei Hans have their own layout of HLA class II allele frequencies.

Conclusion: These data may serve as normal reference values for the Han population in south China.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Asian People / genetics*
China
Gene Frequency*
Genes, MHC Class II*
Humans
Polymerase Chain Reaction
Polymorphism, Genetic
Polymorphism, Restriction Fragment Length