Abstract
Wingless (Wg) is a member of the Wnt family of growth factors, secreted proteins that control proliferation and differentiation during development. Studies in Drosophila have shown that responses to Wg require cell-surface heparan sulphate, a glycosaminoglycan component of proteoglycans. These findings suggest that a cell-surface proteoglycan is a component of a Wg/Wnt receptor complex. We demonstrate here that the protein encoded by the division abnormally delayed (dally) gene is a cell-surface, heparan-sulphate-modified proteoglycan. dally partial loss-of-function mutations compromise Wg-directed events, and disruption of dally function with RNA interference produces phenotypes comparable to those found with RNA interference of wg or frizzled (fz)/Dfz2. Ectopic expression of Dally potentiates Wg signalling without altering levels of Wg and can rescue a wg partial loss-of-function mutant. We also show that dally, a regulator of Decapentaplegic (Dpp) signalling during post-embryonic development, has tissue-specific effects on Wg and Dpp signalling. Dally can therefore differentially influence signalling mediated by two growth factors, and may form a regulatory component of both Wg and Dpp receptor complexes.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Animals, Genetically Modified
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Cloning, Molecular
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Drosophila / genetics
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Drosophila / physiology*
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Drosophila Proteins*
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Epidermis / embryology
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Epidermis / physiology
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Female
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Genes, Insect
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Genetic Techniques
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Genitalia / embryology
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Glycosylphosphatidylinositols / chemistry
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Glycosylphosphatidylinositols / physiology
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Heparan Sulfate Proteoglycans / chemistry
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Heparan Sulfate Proteoglycans / physiology
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Homeodomain Proteins / physiology
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Insect Proteins / physiology
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Larva / chemistry
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Male
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Membrane Glycoproteins / chemistry
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / physiology*
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Mutation
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Proteoglycans / chemistry
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Proteoglycans / genetics
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Proteoglycans / physiology*
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Proto-Oncogene Proteins / physiology*
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RNA / metabolism
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Signal Transduction*
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Transcription Factors / physiology
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Wnt1 Protein
Substances
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Drosophila Proteins
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En protein, Drosophila
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Glycosylphosphatidylinositols
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Heparan Sulfate Proteoglycans
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Homeodomain Proteins
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Insect Proteins
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Membrane Glycoproteins
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Proteoglycans
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Proto-Oncogene Proteins
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Transcription Factors
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Wnt1 Protein
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dally protein, Drosophila
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dpp protein, Drosophila
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wg protein, Drosophila
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RNA