This work describes a novel method to prepare liposomal amikacin composed of soyabean lecithin and cholesterol; these were also prepared using two other methods (cast film method and proliposome method). Encapsulation efficiency was evaluated. Liposomes prepared by the new method, which combines the method of preparing proliposomes with freeze-drying, had the highest encapsulation efficiency. The influence of drug to lipid ratio on the encapsulation efficiency was investigated. The in vitro efflux of amikacin from liposomes with different lecithin: cholesterol ratios was also investigated.