Abstract
Aim:
To study the effect of corticotropin (Cor) on c-fos expression induced by formalin in spinal cord of rat and the role of receptors.
Methods:
Using immunohistochemistry and adrenalectomy.
Results:
Cor inhibited the formalin-evoked c-fos expression in rat spinal cord in a dose-dependent manner. No obvious effect was seen by i.p. Cor 10 U.kg-1, but 25 U.kg-1 reduced the evoked c-fos expression, that was blocked by phentolamine, naloxone, or verapamil, but not much changed by adrenalectomy.
Conclusion:
The formalin-evoked c-fos expression of rat spinal cord was suppressed by Cor through the alpha-adrenergic receptors, opiate receptors and calcium, but no relation to adrenal glands.
MeSH terms
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Adrenalectomy
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Adrenergic alpha-Antagonists / pharmacology
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Adrenocorticotropic Hormone / pharmacology*
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Animals
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Calcium Channel Blockers / pharmacology
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Formaldehyde / antagonists & inhibitors
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Naloxone / pharmacology
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Narcotic Antagonists / pharmacology
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Phentolamine / pharmacology
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Proto-Oncogene Proteins c-fos / biosynthesis*
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Rats
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Rats, Wistar
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Receptors, Adrenergic, alpha / metabolism*
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Receptors, Opioid / metabolism*
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Spinal Cord / metabolism*
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Verapamil / pharmacology
Substances
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Adrenergic alpha-Antagonists
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Calcium Channel Blockers
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Narcotic Antagonists
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Proto-Oncogene Proteins c-fos
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Receptors, Adrenergic, alpha
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Receptors, Opioid
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Formaldehyde
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Naloxone
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Adrenocorticotropic Hormone
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Verapamil
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Phentolamine