Effects of UV-C irradiation on phosphoinositide turnover in plant cells: similarities with those occurring via the formation of reactive oxygen intermediates in animal cells

Comp Biochem Physiol B Biochem Mol Biol. 1999 Mar;122(3):293-9. doi: 10.1016/s0305-0491(99)00018-8.

Abstract

With the aim of examining the response of plant cells to UV-C irradiation, we investigated the behaviour of the phosphatidylinositol 4,5 bisphosphate (PtdIns 4,5-P2) molecule (the precursor of the phosphoinositide signal transduction cascade) by exposing callus cells from Peucedanum verticillare to UV-C (130 J m-2) and by examining the level and the fatty acid composition of PtdIns 4,5-P2 at different times after irradiation. We show that a pathway for the UV-C response includes transient PtdIns 4,5-P2 breakdown. The effect of ultraviolet rays is mimicked by H2O2 suggesting that in this plant it may be brought about by reactive oxygen intermediates (ROI), as already underlined in experimental animal models.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Fatty Acids / analysis
  • Hydrogen Peroxide / pharmacology
  • Phosphatidylinositol 4,5-Diphosphate / metabolism
  • Phosphatidylinositols / metabolism*
  • Phosphatidylinositols / radiation effects
  • Plant Cells
  • Plants / drug effects
  • Plants / metabolism*
  • Plants / radiation effects*
  • Reactive Oxygen Species / metabolism*
  • Ultraviolet Rays

Substances

  • Fatty Acids
  • Phosphatidylinositol 4,5-Diphosphate
  • Phosphatidylinositols
  • Reactive Oxygen Species
  • Hydrogen Peroxide