Design and semisynthesis of spermine-sensitive Ribonuclease S'

I Hamachi; Y Yamada; R Eboshi; T Hiraoka; S Shinkai

doi:10.1016/s0960-894x(99)00189-4

Design and semisynthesis of spermine-sensitive Ribonuclease S'

Bioorg Med Chem Lett. 1999 May 3;9(9):1215-8. doi: 10.1016/s0960-894x(99)00189-4.

Authors

I Hamachi¹, Y Yamada, R Eboshi, T Hiraoka, S Shinkai

Affiliation

¹ Department of Chemistry & Biochemistry, Graduate School of Engineering, Kyushu University, Hakozaki, Fukuoka, Japan.

PMID: 10340601
DOI: 10.1016/s0960-894x(99)00189-4

Abstract

Spermine-sensitive stabilization of semisynthetic Ribonuclease S' was successfully carried out by sequence specific incorporation of a poly-anion domain into alpha-helix region of S-peptide.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Amino Acid Sequence
Kinetics
Models, Chemical
Models, Molecular
Molecular Sequence Data
Protein Binding
Protein Denaturation
Protein Structure, Secondary
Ribonucleases / chemical synthesis*
Ribonucleases / chemistry*
Spermine / chemistry*
Temperature

Substances

Spermine
Ribonucleases
ribonuclease S